WBCs

• The Basics
• Reactive
• Leukemic

• The Basics
• Diseases

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Quiz Please

• First, the RBCs are lysed, leaving only the WBCs.
• The WBCs are diluted with a buffer so they can be shot,  single-file, through a flow cell. 
• The white blood cells are sized as they pass through a tiny window in the flow cell. 
• The machine registers an impedance change across the flow cell window proportional to the size of each WBC.
• The complexity of each WBC is assessed by laser light light absorbance and reflection.
• Nuclear to cytoplasmic ratio.
• Degree of granularity of the cyotplasm.
• Absorbance characteristics of the granules.
• Want see a movie of how it's done?

What do the areas of the scatter plot correspond to?       Back