It's easy to make mistakes and introduce artifacts when making a slide, therefore each of the foregoing steps must be done with great care.

Give the major purpose of each of these steps in tissue preparation for light microscopy.

• Fixation
• Removing the water in dehydration
• “Clearing” with xylene or other organic solvent
• Embedding with paraffin
• Sectioning with a microtome
• Staining

The histological primary stain.